Ex2 Fluorescence

Wed, 06/05/2013 - 08:24 -- sewm02

Ex2 Fluorescence

 

Question 1 Diagram a fluorometer, be sure to indicate how each and every part of the fluorometer works and what it consists of as appropriate (Will and Ian)

Reference Question 1 (Will and Ian)

 Skoog, D. A., Holler, F.J., and S.R. Crouch. Principles of Instrumental Analysis 6th Edition. Canada: Thomsom Brooks/Cole, 2007.

Annotation Question 1 (Will and Ian)

 A nice diagram is found on page 415 which can be found in the reserved section of the library. This source is reliable because it comes from a text book. This reference can also provide a baseline answer for all the fluorometric questions presented for the test.

 

Question 1A Commonly used continuous wave sources (Will and Ian)

Reference Question 1A (Will and Ian)

PTI Website

Annotation Question 1A (Will and Ian)

We accessed this site on January 27th 2008. The website used is from the company that made our fluorometer in our lab. It lists the specifications for all the fluorometers that it currently makes. Along with this it list the most common types of lamps used a continuous waves source. We feel it is reliable because it is provided through a reputable company.

 

Question 1C Diagram a monochromator that uses a diffraction grating (Will and Ian)

Reference Question 1C (Will and Ian)

Harris, D.C. Quantitative Chemical Analysis 7th Ed. New York: W.H. Freeman and Company, 2007. (Page 429) Skoog, D. A., Holler, F.J., and S.R. Crouch. Principles of Instrumental Analysis 6th Edition. Canada: Thomsom Brooks/Cole, 2007. (Page 182 - 184)

Annotation Question 1C (Will and Ian)

On page 429 there is a detailed diagram showing the working of a diffraction grating. This source is reliable because it is our textbook. The second reference explains in detail how the grating is capable of focusing the light. It also goes into detail on a few varying types of grating. Again this book is on reserve in the library.

 

Question 1D How Does a photomultiplier work? (Will and Ian)

Reference (Will and Ian)

Skoog, D. A., Holler, F.J., and S.R. Crouch. Principles of Instrumental Analysis 6th Edition. Canada: Thomsom Brooks/Cole, 2007. (PG 194-195)

Annotation (Will and Ian)

There is a detailed diagram of the photomultiplier tube along with a detailed section outlining how the tube works.

 

Question 1E What are the optics made of? (Will and Ian)

Reference (Will and Ian)

PTI Website

Annotation (Will and Ian)

This reference again comes from a company that produces many high quality fluorometers, including the one that we currently have at the school. It list the type of optics used in their machines.

 

Question 2 Energy Diagram of Fluoresence (Will and Ian)

Reference (Will and Ian)

Harris, D.C. Quantitative Chemical Analysis 7th Ed. New York: W.H. Freeman and Company, 2007. (Page 388-391) Skoog, D. A., Holler, F.J., and S.R. Crouch. Principles of Instrumental Analysis 6th Edition. Canada: Thomsom Brooks/Cole, 2007. (Page 153-156)

Annotation (Will and Ian)

Both these references provide very similar material with a relatively high level of overlap, but one will find them both extremely fun to review. The diagrams are quite vividly displayed in the typical blue color preferred by all chemistry text books. All material related to fluorescence and phosphorescence can be found in both books. You will also be able to answer the sub question listed on the test sheet.

 

Question 3 What is the relationship between concentration and fluorescence? (Will and Ian)

Reference (Will and Ian)

Truman State Chem Lab Skoog, D. A., Holler, F.J., and S.R. Crouch. Principles of Instrumental Analysis 6th Edition. Canada: Thomsom Brooks/Cole, 2007. (Page 407)

Annotation (Will and Ian)

A good general resource that covers many aspects of using a fluorometer. While not going into great detail this site is able to greatly enhance you basic knowledge of fluorometry. Since it comes from a university

website we feel the information is reliable.

 

Question 4 Emmision Spectra and Excitation Spectrum (Will and Ian)

Reference (Will and Ian)

Harris, D.C. Quantitative Chemical Analysis 7th Ed. New York: W.H. Freeman and Company, 2007. (Page 394)

Annotation (Will and Ian)

Covers the basic terminology needed to understand and answer the question, while also providing graphs to assist in visually depicting the event.

 

Question 5 What is meant by quenching? (Will and Ian)

Reference (Will and Ian)

Skoog, D. A., Holler, F.J., and S.R. Crouch. Principles of Instrumental Analysis 6th Edition. Canada: Thomsom Brooks/Cole, 2007. (Page 408-409)

Annotation (Will and Ian)

Talks about how quenching works and outlines it with an energy diagram.